Effect Of Arbutin Peel From Pyrus Communis On Cell Line

Abstract

Arbutin (P-Hydroxyphenyl-Β-D-Glucopyranoside) Possesses Beneficial Extracted From Plant Pyrus Communis  Functions Including Anti-Tumor Activities. Due To The Important Role Of Anti Tumor And Apoptosis In The Successful Treatment Of Cancer, Is Essential. The Purpose Of The Current Study Was To Evaluate The Effect Of Arbutin On Cell Line Hepg2 ,MCSF7 And MEF Cellline .                                                                                                              

Materials And Methods  In This Experimental Study, Effect Arbutin And Extracted From Pyrus Communis  In  Hepg2 ,MCSF7  And MEF Cell Lines Were Pre-Treated With Arbutin (6.12 ,12.5, 25,50, And 100 Μm). After 24 Hours, T-BHP (30 And 35 Μm) Was Added To The Cells. Viability Was Measured (At 24) Using MTT Assay (3-[4,5-Dimethylthiazol-2-Yl]-2,5 Diphenyl Tetrazolium Bromide).                                                                                                  

Results: Arbutin Treatment To Cell Line Assay Including Effect Of Extract On Growth Of Tumor Cell Lines Hepg2, MCF7 And MEF Normal Cell Line Was Done By Using Cytotoxic Assay. The Findings Demonstrated That The Cytotoxicity Of The Extract Varied Depending On Cell Type And Concentration In Different Cell Lines. With Higher Extract Concentrations, Tumor Cell Line Inhibition Activity Increased. The Higher Inhibition Rate In Hepg2  Cell Line Was 92.01 %  And 79.87 At The Concentration 100 µg\Ml While For MCF7  The Highest Inhibition Rate Was 62.12% And 59.87in Concentration 100 µg\Ml The Highest Inhibition Rate For MEF  Cell Line Was 59.17 %  And 42.81 In 100µ G\Ml

Conclusion: Arbutin As A Potential Functional And Extracted  Has Strong Ability To Inhibitory Effect On The Growth Of  Cell Line And Decreased Effect In Normal Cell Line .