Repairing Rabbit Femur Bone Defects with Graphene Oxide Scaffolds and Gold Nanoparticles

Abstract

Background :The byproduct of graphite oxidation is graphene oxide, which offers a tremendous chance to advance the field of regenerative medicine significantly. Because of graphene oxide's favorable qualities— Because of its adaptable mechanical qualities, biocompatibility, and degradability. Objective: This research aims to employ laser rays to ablate the graphite surface and prepare graphene oxide with excellent mechanical properties that contribute to the treatment of rabbit bone injuries, regeneration of damaged bone tissue and osteogenic differentiation .Methods: In this work, the xeno bony scaffolds implantation  were prepared in two parts physically: were effectively generated graphene oxide(GO) by using laser ablation technique .Lasing pulse duration(1064 nm) with laser energy (300 mJ), after that, gold nanoparticles(AuNPs) prepared previous were loaded on the graphene oxide surface by using chips method. The structure and morphology of the composite (GO: AuNPs) were analyzed through Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD) to assess its chemical composition and crystalline structure. Raman spectroscopy and transmission electron microscopy (TEM) were then used to examine the edge/crystalline orientations and stacking orders of graphene oxide.: In this section, the technique of preparing a pin template to anchor the scaffold implantation into the rabbits' stimulated femoral bone fractions is described. A combination made of Graphene oxide and gold nanoparticles were utilized  to create the xeno bony scaffold. Results. The histological results in vivo of the site treated with (GO: AuNP) scaffolds, harvested at 1st week after grafting showed bone mineralization and abundant new bone formation without any sign of inflammation in treatment group. Moreover, the in vitro tests demonstrate that the (GO: AuNP) scaffolds greatly enhance osteoblast adhesion cells as well as growth. Conclusion :the application of graphene oxide (GO) enhanced calcium and protein adsorption, enzyme resistance, and physical strength.