Detection of sul1 resistance gene in Acinetobacter baumannii from different Clinical cases

Abstract

Acinetobacter baumannii, is widespread, opportunistic pathogen that has conceded as a global threat because of high levels of resistance to many antibiotics. Many study report the molecular characterization of sulphonamide resistance genes and gene cassettes associated with class I integrons in various Enterobacteriaceae including extended spectrum b-lactamase (ESBL) producers. sul1, sul2 and sul3 encoding dihydropteroate synthases and more than 20 dihydrofolate reductase (dfr) genes have been described. Both groups of genes are associated with class 1 integrons residing in plasmids and/or the bacterial chromosome.

Nineteen isolates of Acinetobacter baumannii were obtained from various clinical cases including sputum (8), blood (5), wound swab (4), urine (1) and throat swab (1).

The antimicrobial susceptibility results revealed that Acinetobacter baumannii isolates were resistance to Ceftazedim, Ciprofloxacin, Cefotaxim, Cefepime, Ampicillin and Agumantin (100 %), gentamicin (94.7%), and Imipenim (68.4%).

 Molecular investigation of (sul1) gene exhibited that (sul1) gene was detected in 17 (98%) of isolates. all isolates was multidrug resistance.  In other hand, sul1 gene was not detected in strains 5, 13 only and both of them were resistant to 7 antibiotics and sensitive to Imipenem. The gel electrophoresis revealed that the molecular weight of the (sul1) gene was (822) bp.