Evaluation of the antibacterial and antioxidant activity of Alternaria chlamydospora extract isolated from the soil.

Abstract

The study included isolate and diagnose fungus Alternaria chlamydospora of the local soil and purified and development in the PDB medium and the filtrate extracted using a solvent (ethyl acetate) to obtain the fungal extract. This extract has shown bioactivity against both reference isolates (E.coli (ATCC25922) and S.aureus (NCTC6571)) and pathogenic isolates S.pyogenes ,K. pneumonia and Ps.aeruginosa using agar disk diffusion   technique , The diameters of the inhibition zones of fungal extract  31.5 mm against S.aureus and 29.5 mm against E.coli,  and 30.0 mm against K.pneumoniae and 17.0 mm against S.pyogenes While isolation was not affected Ps.aeruginosa. The test revealed the minimum inhibitory concentration (MIC) of the fungal extract less inhibitor concentration towards our isolation S.aureus was 3.12 µg/ ml also less deadly concentration (MBC).Also against isolate S.aureus was 6.25µg/ Ml. also fungal extract showed no toxicity toward human red blood cells. Chemical statements to fungal extract showed a contain on Tannins and phenols, and it contains amino acids and flavonoids. Tested the antioxidant  of the fungal extract by measuring its ability to be reduced iron duo ions (Fe Π) have shown high rates of inhibition. The metabolic product of soil fungi can be an alternative source of commercial anti-bacterial.