Study of Multiple resistance Pattern to antibiotic of clinical and Swege E. coli isolates and detection of blaIMP-1 gene by using PCR.

Abstract

     During a 4 months period a dominated pattern of multiple resistance to antibiotic of clinical E. coli isolates which isolated from Central Medicine City hospital / Baghdad ,were analyzed and compared with the multiple resistance pattern of sweges isolates .the results revealed that the multiple resistance pattern of clinical isolates was    :  CX - CRO – CE- AMC - TR  and the pattern of sweages was : CX – CRO- CE- AMC.

   The isolates which resistant to Cefotaxime , Ceftazidime and Imipenem were selected to detect the ability to  Metallo ß -lactamases production (MßLs) .Ten clinical isolates and five swege isolates were able to produce MßLs ,and these isolates were tested to bla_IMP-1   by using polymerase chain reaction( PCR) and using Primer :

  5- ACC GCA GCA GAG TCT TTG CC – 3                  ( F)

5-ACA ACC AGT TTT GCC TTA CC  _ 3                    (R  )  

             Two isolate appeared to carry bla_IMP-1 gene after electrophoresis of the results on 1.2% agarose.

   The results of Bacterial Conjugation experiment between Clinical and Swege isolates showed that the Metallo β-Lactamases were unable to transferred in this process.