Relationship study between the alkaline protease production and the growth phases of Pseudomonas aeruginosa isolated from patients

Abstract

      This study was conducted  in Ba’quba general hospital and Al-Batul hospital during 1/12/2004 to 31/3/2005.Occurrence of Pseudomonas aeruginosa was investigated in 161 samples from different clinical sources included Swabs from wounds, burns, ear, eye and samples from Urine and sputum. Which were collected from patients.

        Depending on the cultural and micro features and biochemical tests, 49 isolated items of this bacteria have been diagnosed and all the isolates showed the ability to produce alkaline protease enzyme by using skim milk agar medium through forming clear zone around the growing colonies.

      The local isolate P.aeruginosa AP3  had been selected based on the higher productivity of enzyme comparing to other isolated and thus it was used in the current study.

     The relationship between the production of alkaline protease enzyme   and growth phases of  P.aeruginosa was studied to determine the time  of production of the enzyme . The results showed that the local isolate P.aeruginosa AP3 began the production of the enzyme in the later stages in the log phase and it was increased significantly in the stationary phase reaching the maximum after 48 hours (159.2 units/ml) in leaky farm and keep the enzyme was fully functional 'almost in the stationary phase.