Isolation of a Novel Brevibacillus parabrevis PS12 Strain from Soil and Determination of Optimal Conditions for Arginase Production

Abstract

       Soil-occupy Brevibacillus parabrevius PS12 species are known for their metabolically versatile and it is being discovered as source of natural enzymes valuable to industry. Arginase catalyzes the processing of L-arginine to L-ornithine and urea, it is the one that plays a significant role in nitrogen metabolism Finding novel bacterial strains from environmental sources that are capable of producing the arginase enzyme is crucial for expanding microbial resources for industrial and pharmaceutical use. Among the 20 isolates, one isolate was identified as Brevibacillus parabrevis PS12 (97% 16S rRNA similarity, GenBank accession no. (PX118439.1), representing the first report from Iraqi soil. Arginase activity reached 8.3 U/mg protein a peak in nutritional source with maltose as a arbone source  and casein as a Nitrogen source, the physical parameters also effect on enzyme specific activity  at 7.0 pH, 37°C, and 48 hours of incubation time with shaker incubator 150 rpm to ensure the homogeneity of all medium components and continuous aeration all these factors contributed to an increase in bacterial biomass which led to a rise in the specific activity of arginase.