Effect of pomegranate Extract and luteolin on Gram Negative and Positive Bacteria

Abstract

 The antibacterial effect of rind of pomegranate extract and Luteoline on the growth was evaluated  in vitro serial dilution method was used to the growth of various Gram-negative and Gram-positive bacteria. The bactericidal activity of this extract was analyzed by serial dilution in tubes. In this study it was found that Gram positive bacteria are susceptible to very low pomegranate and luteoline concentrations. On the other hand, Gram negative bacteria were more resistant, with  minimal bactericidal concentration of this extract ranging between 5 - 10 mg ml^-1 pomegranate and 2,4 mg ml^-1  of luteoline , for Gram-positive bacteria and were ranging between 10 - 20 mg ml^-1 pomegranate and 4,8 mg ml^-1  of luteoline for  Gram-negative bacteria. The results of this study suggest that crude extraction of pomegranate and luteoline is effective in controlling of many diseases  as a natural resource to prevent many bacterial infections.