تقدير المحتوى البروتيني في بذور نبات الكتان Linum Usitatissimum والنباتات الناتجة من الزراعة النسيجية

الملخص

Callus induction from different explant in Linum usitatissimum in MS medium Provided by variable concentration of growth regulators, cotyledons and hypocotyls have show high response to form callus in MS that contains (BA, NAA) with (1.0) mg/liter concentration for each one. It was same for MS that contains (Kin, 2,4-D) with (2.0) and (1.0) mg/liter respectively. The result of Protein estimate in seeds, explants and callus showed that the rate of Protein in seeds about (0.30) mg/g, and in explant the high rate of Protein in Leaf about (0.50) mg/g, followed by hypocotyls about (0.45) mg/g while in Root was about (0.10) mg/g. In callus the high Protein rate in Leaf callus about (0.35) mg/g then in hypocotyls about (0.30) mg/g and the minimum rate showed in root callus about (0.10) mg/g.