Study the optical condition and effect of Ultraviolet ray on extracellular polysaccharide pullulans production from local isolation of Alternaria alternate

الملخص

Six local isolates of Alternaria alternate were isolated from different infection plant.The ability of these isolates for pullulans production using production medium was investigated.The highest production of biomass and pullulans were achieved from isolates [A3] with (15.88) g/L and then isolates [A1] gave (13.12) g/L from pullulans.Experiment has affected the exposure time of ultraviolet light on isolates strains [A1,A3].The results are showed isolates [A1,A3] gave highest of production from the first case (not exposure to ultraviolet light) with [17.81,19.81] g/L respectively,studied effect different carbon,nitrogen sources to strain [A3] it gave highest production the pullulans,the result showed to sucrose as a carbon sources and ammonium phosphate as a nitrogen sucrose wereyielded the best production of pullulans (20.43,20.06)g/L at six day of incubate.