Study of optimal conditions for invertase production from a local isolate of Candida spp

Abstract

Numbers of yeast isolates from different sources were subjected to primary  screening to recognize  which one of them having   the ability for invertase  production  . Five of them which related to Candida can produce invertase . The isolate which was designated C5 was highest producer among them and identified as Candida spp.. Results showed that the optimum condition for production of the enzyme from this isolate  by submerged culture was achieved using a media containing rafinose as a carbon  source with concentration of 3% and a mixture of yeast extract : ammonium sulfate 1:1  with 0.4 %  concentration as nitrogen source. The initial pH  was 6 .  The duration and  temperature of incubation were 28h and  35 C˚  respectively. The enzyme activity of invertase under these conditions was 1.58 unit /ml with a specific activity of 6.07 unit/mg.